Koide Laboratory - Synthetic Protein Science

We aim to advance the knowledge of how proteins and protein interaction networks work by “synthetic” approaches, i.e. designing and making proteins with novel function.  This "learning by building" approach critically tests our understanding of principles that govern protein structure, function and evolution, and it challenges our creativity.

We are now capable of producing highly functional synthetic proteins within weeks.  Structural and functional analysis of synthetic proteins not only critically evaluates the effectiveness of our design strategies but also provides broader insights into the molecular mechanism and evolution of protein form and function.  Knowledge gained from the synthetic approach complements and very often significantly expands the view from classical studies of natural proteins.

Synthetic proteins serve as novel and transformative tools for basic and translational research in medicine and beyond.  We are particularly interested in applying our capability to:

  • Development of biologics therapeutics (engineered proteins and antibodies)
  • Signal transduction and cancer biology
  • Epigenetics
  • Structural biology
  • Synthetic biology

Why synthetic protein science?


July 2018 - Kevin is awared an NIH Kirschstein-NRSA postdoctoral fellowship. Congrats!

July 2018 - Our paper on drug target validation using monobides is published in Nature Chemical Biology.

July 2018 - Our paper on the monobody-assisted crystal structure of CLC-type fluoride/proton antiporter is published in Nature Struct Mol Biol.

May 2018 - Our paper on modulating lipase specificity with proxy monobodies is published in ACS Chemical Biology.

May 2018 - Our review on the next-generation PTM antibodies is published in Current Opinion in Structural Biology.

January 2018 - Our paper on the monobody-assisted crystal structure of ICMT is published in Nature.

December 2017 - Linxia is awared an American Cancer Society postdoctoral fellowship. Congrats!

December 2017 - Our collaboration paper on the DH-PH domains of Bcr-Abl is published in Nature Commun.

September 2017 - Gabe's paper on monobodies that activate or inhibit GPR56 is published in PNAS.

July 2017 - Gabe successfully defended his Ph.D. Congrats Dr. Salzman!

March 2017 - Our review on Monobody and other synthetic binding proteins is published in Protein Science (open access).