Koide Laboratory - Synthetic Protein Science

We aim to advance the knowledge of how proteins and protein interaction networks work by “synthetic” approaches, i.e. designing and making proteins with novel function.  This "learning by building" approach critically tests our understanding of principles that govern protein structure, function and evolution, and it challenges our creativity.

We are now capable of producing highly functional synthetic proteins within weeks.  Structural and functional analysis of synthetic proteins not only critically evaluates the effectiveness of our design strategies but also provides broader insights into the molecular mechanism and evolution of protein form and function.  Knowledge gained from the synthetic approach complements and very often significantly expands the view from classical studies of natural proteins.

Synthetic proteins serve as novel and transformative tools for basic and translational research in medicine and beyond.  We are particularly interested in applying our capability to:

  • Development of biologics therapeutics (engineered proteins and antibodies)
  • Signal transduction and cancer biology
  • Epigenetics
  • Structural biology
  • Synthetic biology

Why synthetic protein science?

News

August 2020 - STAT3 Monobody paper with Prof. O. Hantschel's group is published in Nature Comm.

April 2020 - Our paper with Prof. J. Murphy's group on dissection of the necroptosis signaling pathway with Monobodies targetting MLKL is published in PNAS.

Feb 2020 - Our review on the utility of the Monobody technology in structural and mechanistic biology is published in Current Opinion in Structural Biology.

August 2019 - Shohei's commentary is published in PNAS.

July 2019 - Our paper on the development of Monobodies that allosterically activate and inhibit Aurora A kinase is published in PNAS.

December 2018 - Kevin is awarded an American Cancer Society postdoctoral fellowship. Congrats!

July 2018 - Kevin is awarded an NIH Kirschstein-NRSA postdoctoral fellowship. Congrats!

July 2018 - Our paper on drug target validation using Monobides is published in Nature Chemical Biology.

July 2018 - Our paper on the Monobody-assisted crystal structure of CLC-type fluoride/proton antiporter is published in Nature Struct Mol Biol.

May 2018 - Our paper on modulating lipase specificity with proxy Monobodies is published in ACS Chemical Biology.

May 2018 - Our review on the next-generation PTM antibodies is published in Current Opinion in Structural Biology.

January 2018 - Our paper on the Monobody-assisted crystal structure of ICMT is published in Nature.